中国药理学通报2026，Vol.42Issue(10)：1993-1998,6.DOI:10.12360/CPB202608006
中性粒细胞条件性敲除Hif-1α基因小鼠的构建和鉴定
Construction and identification of neutrophil-specific conditional Hif-1α gene knockout mice
摘要
Abstract
Aim To construct neutrophil-specific conditional hypoxia-inducible factor 1α(HIF-1α)knockout mice and ana-lyze their genotypes,providing an animal model for studying the function of HIF-1α in neutrophils.Methods Hif-1αflox/+mice were crossed with Ly6g-iCre mice to obtain Hif-1αflox/+Ly6g-iCre+mice,which were then intercrossed to generate Hif-1αflox/floxLy6g-iCre+target mice.Genomic DNA was extracted,and genotypes were identified by PCR amplification followed by agarose gel electrophoresis.H&E staining was used to evaluate the effects of neutrophil-specific knockout on major organs including the heart,liver,spleen,lungs,and kidneys.Western blot was per-formed to detect HIF-1α protein expression in bone marrow neu-trophils and peripheral blood neutrophils under hypoxic condi-tions.Results Genotyping results confirmed the successful construction of Hif-1αflox/floxLy6g-iCre+mice.Conditional dele-tion of HIF-1α in neutrophils did not affect mouse growth,de-velopment,or reproduction.Western blot results showed that HIF-1α expression was significantly reduced in both bone mar-row and peripheral neutrophils of Hif-1αflox/floxLy6g-iCre+mice under hypoxic conditions.No obvious pathological changes were observed in major tissues and organs including the heart,liver,spleen,lungs,and kidneys.Conclusion Neutrophil-specific conditional HIF-1α knockout mice are successfully con-structed and identified,providing an experimental animal model for further investigation of the role of HIF-1α in neutrophil-related diseases.关键词
中性粒细胞/缺氧诱导因子1α/细胞条件性敲除/Cre-Loxp重组酶系统/基因鉴定/小鼠模型Key words
neutrophils/hypoxia-inducible factor 1α/condi-tional knockout/cre-LoxP recombinase system/genotyping/mouse model分类
医药卫生引用本文复制引用
马云潇,柏梦琳,付梦露,程轩,吴涵,张贤政,张玲玲..中性粒细胞条件性敲除Hif-1α基因小鼠的构建和鉴定[J].中国药理学通报,2026,42(10):1993-1998,6.基金项目
国家自然科学基金资助项目(No 82204403) （No 82204403）
中国博士后面上项目(No 2022M72199) （No 2022M72199）
安徽医科大学临床医学"5+3"一体化专业"早期接触科研"训练计划项目(No 2025-ZQKY-110) （No 2025-ZQKY-110）
