电子科技2026，Vol.39Issue(10)：87-94,8.DOI:10.16180/j.cnki.issn1007-7820.2026.10.010
基于预标定内参的数字体视显微系统标定方法
Calibration Method for Digital Stereoscopic Microscopy System Based on Pre-Calibrat-ed Intrinsic Parameters
摘要
Abstract
In view of the problem that the shallow depth of field of the digital stereomicroscope system makes the general camera calibration method inapplicable,this study proposes a calibration method for the digital stereomi-croscope system based on the pre-calibrated internal reference based on the Zhang Zhengyou calibration method.By using the variable magnification method and the equivalent model of the microscope to pre-calibrate the princi-pal point and focal length,the internal parameters of the camera are accurately estimated.By combining these inter-nal parameters with Zhang Zhengyou's calibration method to estimate and optimize other camera parameters,the cal-ibration of the logarithmic stereoscopic microscopic system in the microscopic environment is achieved.The experi-mental results show that the pre-calibrated internal reference method can stably and accurately obtain the principal point and focal length,providing reasonable initial values for the optimization of camera parameters.In the calibra-tion of the actual digital stereomicroscope system,the proposed method can accurately calibrate the camera parame-ters under different commonly used magnifications.The reprojection error is less than 0.5 pixel,and the optical ax-is angle is 9.6°,which is close to the equipment calibration parameter 10°.When conducting depth measurement experiments using the calibrated camera parameters,the average relative error of multiple measurements at 50 μm is 2.29%,and the mean square error is 1.5 μm,verifying the effectiveness and reliability of the proposed calibra-tion method.关键词
显微立体视觉/体视显微镜/相机标定/张正友标定法/机器视觉/单目标定/极线校正Key words
micro stereo vision/stereo microscope/camera calibration/Zhang Zhengyou calibration meth-od/machine vision/single targeting/epipolar rectification分类
信息技术与安全科学引用本文复制引用
陈泽,袁嫣红,乐珂..基于预标定内参的数字体视显微系统标定方法[J].电子科技,2026,39(10):87-94,8.基金项目
浙江省科技厅项目(2022C01065) Zhejiang Science and Technology Department Project(2022C01065) （2022C01065）
