分析化学2026，Vol.54Issue(9)：1588-1600,13.DOI:10.19756/j.issn.0253-3820.261222
微塑料与重金属复合胁迫对谷胱甘肽抗氧化活性的影响及机理分析
Effects of Combined Stress of Microplastics and Heavy Metals on Glutathione Antioxidant Activity and Mechanistic Analysis
摘要
Abstract
To investigate the combined effects of microplastics and heavy metals on the antioxidant function of glutathione(GSH),a simplified in vitro free radical scavenging model was established using GSH and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical.The influences of polyethylene terephthalate(PET),polystyrene(PS),and polypropylene(PP)coexisting with varying concentrations of As(Ⅲ),As(Ⅴ),Cd2+,and Pb2+on the DPPH radical scavenging efficiency of GSH were studied.Dynamic changes in the relative contents of GSH and oxidized glutathione(GSSG)in the arsenic-containing systems were monitored by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry(HPLC-ESI-MS).The results demonstrated that the promoting effects of As(Ⅴ),Pb2+and Cd2+on DPPH scavenging activity of GSH decreased progressively as their relative concentrations decreased,whereas the effect of As(Ⅲ)exhibited an initial increase followed by a subsequent decline.Under a high microplastic content condition(Ratio of reaction solution to microplasticsmLiquid:mMPs=1:2),the aged microplastics consistently enhanced the free radical scavenging rate.In the closed reaction system,a certain molar ratio of GSH/As(Ⅲ)within the range of 1:2 to 2:1 was most favorable for the generation of GS·or GSSG,thereby accelerating the radical scavenging reaction rate.The reduction and complexation reactions of As(Ⅴ)competitively consumed GSH in the DPPH radical scavenging process,resulting in a negative correlation between GSSG generation and As(Ⅴ)concentration,as well as a lagged change in GSH content.关键词
微塑料/重金属胁迫/谷胱甘肽/抗氧化活性/复合效应Key words
Microplastics/Heavy metal stress/Glutathione/Antioxidant activity/Composition effect引用本文复制引用
汪明烨,王天天,张昊顺,蒋凤华,孙承君,李景喜..微塑料与重金属复合胁迫对谷胱甘肽抗氧化活性的影响及机理分析[J].分析化学,2026,54(9):1588-1600,13.基金项目
南亚防灾减灾能力建设合作项目(No.WJ1526003)和亚洲合作基金项目(No.WJ1326013)资助. Supported by the Cooperations on Capacity Building for Marine Disaster Prevention and Mitigation in South Asia(No.WJ1526003)and the Asian Cooperation Fund(No.WJ1326013). （No.WJ1526003）
