农业与技术2026，Vol.46Issue(10)：1-6,6.DOI:10.19754/j.nyyjs.20261030001
玉竹根尖离体培养及芽增殖技术优化研究
Optimization of In Vitro Root Tip Culture and Shoot Multiplication Technology for Polygonatum odoratum(Mill.)Druce
摘要
Abstract
In this study,young root tips of Polygonatum odoratum(Mill.)Druce were used as explants.The effects of sterilization conditions,explant segments,low-temperature pretreatment,combinations of plant growth regulators,sucrose concentration and medium pH on in vitro culture performance were systematically investigated.The results showed that the optimal sterilization procedure was 75％ethanol treatment for 8 s followed by 0.1％mercuric chloride treatment for 4 min.The middle segments of root tips with a length of about 0.5 cm exhibited a high capacity for adventitious bud induction.Low-temperature pretreatment at 4℃for 6 days significantly promoted bud differentiation.The MS medium supplemented with 0.7 mg·L-1 6-BA and 0.6 mg·L-1 NAA achieved the best shoot proliferation efficiency.A sucrose concentration of 25 g·L-1 and medium pH of 5.8 were conducive to normal bud growth.This study established an efficient in vitro culture and shoot proliferation system using root tip explants of Polygonatum odoratum(Mill.)Druce,which provides a technical reference for the rapid propagation of Polygonatum odoratum(Mill.)Druce seedlings.关键词
玉竹/根尖/组织培养/不定芽诱导/快速繁殖Key words
Polygonatum odoratum(Mill.)Druce/root tip/tissue culture/adventitious bud induction/rapid propagation分类
农业科技引用本文复制引用
吕娇娇,熊胜,刘明新..玉竹根尖离体培养及芽增殖技术优化研究[J].农业与技术,2026,46(10):1-6,6.基金项目
国家新农村发展研究院植物资源开发项目(项目编号:xczx-2023175) （项目编号:xczx-2023175）
