农业与技术2026，Vol.46Issue(10)：7-12,6.DOI:10.19754/j.nyyjs.20261030002
白术愈伤组织诱导及丛生芽增殖培养基优化研究
Optimization of Media for Callus Induction and Cluster Bud Proliferation in Atractylodes macrocephala Koidz
摘要
Abstract
To establish an efficient in vitro regeneration and rapid propagation system for Atractylodes macrocephala Koidz.and solve the problems of low efficiency and cultivar degradation in traditional propagation methods,stems and leaves of aseptic A.macrocephala seedlings were used as explants in this study.Single-factor experiments were conducted to screen suitable conditions for callus induction,and an L9(34)orthogonal experimental design was adopted to systematically optimize the effects of four key factors(6-BA,NAA,TDZ and sucrose)on the differentiation and proliferation of cluster buds,with supporting rooting and transplanting procedures established.The results showed that stem explants performed better than leaf explants in callus induction.The optimal callus induction medium was MS medium supplemented with 2.0 mg·L-1 2,4-D,0.1 mg·L-1 6-BA and 35g·L-1 sucrose,under which the callus induction rate reached 92.50％and the embryonic callus rate reached 81.67％.Range analysis and variance analysis of the orthogonal test indicated that the order of influence of the four factors on the proliferation coefficient of cluster buds was 6-BA>NAA>TDZ>sucrose,among which 6-BA and NAA had significant effects(P<0.05).The optimal differentiation and proliferation medium was MS medium containing 2.0 mg·L-1 6-BA,0.2 mg·L-1 NAA,0.2 mg·L-1 TDZ,35g·L-1 sucrose and 7g·L-1 agar.On this medium,the cluster bud differentiation rate reached 95.56％,the proliferation coefficient reached 6.42,and the average bud length was 2.17 cm,with stout buds,dark green leaves and no vitrification.The optimal rooting medium was 1/2 MS medium supplemented with 0.5 mg·L-1 IBA and 30g·L-1 sucrose,with a rooting rate of 93.33％and an average root length of 3.42 cm.After acclimatization,plantlets were transplanted into a substrate of peat soil and perlite at a volume ratio of 2:1,with a survival rate of 89.17％.This study established a complete in vitro regeneration system for A.macrocephala from callus induction to transplant survival,which can provide technical support for large-scale propagation of elite seedlings and germplasm innovation of A.macrocephala.关键词
白术/愈伤组织诱导/丛生芽增殖/正交试验/生根培养/移栽Key words
Atractylodes macrocephala Koidz./callus induction/cluster bud proliferation/orthogonal test/rooting culture/transplanting分类
农业科技引用本文复制引用
张鹏里,王翔,李健..白术愈伤组织诱导及丛生芽增殖培养基优化研究[J].农业与技术,2026,46(10):7-12,6.基金项目
国家新农村发展研究院植物资源开发项目(项目编号:xczx-2023175) （项目编号:xczx-2023175）
