农业与技术2026，Vol.46Issue(10)：37-44,8.DOI:10.19754/j.nyyjs.20261030007
响应面法优化超声辅助双酶提取人参皂苷的工艺
Optimization of Ultrasonic-Assisted Double Enzymatic Extraction Technology of Ginsenosides by Response Surface Methodology
摘要
Abstract
To optimize the ultrasound-assisted dual enzymatic extraction process of ginsenosides from Panax ginseng,this study first investigated the effects of usage of cellulase and pectinase,enzymolysis temperature,enzymolysis time,solid-liquid ratio,ethanol concentration,extraction time,and ultrasound power on the ginsenoside extraction yield through single-factor experiments.Among these factors,ethanol concentration,enzymolysis temperature,and ultrasound power were identified as the key influencing variables.On this basis,a Box-Behnken design combined with response surface methodology was adopted to establish a regression model between the extraction yield and the selected factors,and subsequently to determine the optimal process parameters.The results showed that the model-predicted optimal conditions were ethanol concentration of 69％,enzymolysis temperature of 61℃,and ultrasound power of 558 W,under which the predicted extraction yield was 60.92 mg·g-1.For practical applicability,the parameters were adjusted to ethanol concentration of 70％,enzymolysis temperature of 60℃,and ultrasound power of 560 W.Under these modified conditions,the actual average extraction yield reached 61.28 mg·g-1,which was in good agreement with the predicted value.This study established a stable and efficient process for extracting ginsenosides,providing a foundation for the high-value utilization of Panax ginseng.关键词
人参皂苷/超声辅助双酶提取/响应面法/工艺优化Key words
ginsenosides/ultrasound-assisted dual-enzyme extraction/response surface methodology/process optimization分类
农业科技引用本文复制引用
袁梦琳,杨飞龙,李洁,邹海丹,刘洋,江和源..响应面法优化超声辅助双酶提取人参皂苷的工艺[J].农业与技术,2026,46(10):37-44,8.基金项目
中国农业科学院创新工程(项目编号:ASTIP2026-34-IUA-09) （项目编号:ASTIP2026-34-IUA-09）
