生物技术通报2026，Vol.42Issue(10)：55-66,12.DOI:10.13560/j.cnki.biotech.bull.1985.2025-1424
基于WGCNA的玉米雄穗耐热相关共表达模块鉴定和核心基因发掘
Identification of Heat-responsive Co-expression Modules and Mining of Hub Genes in Maize Tassel Based on WGCNA
摘要
Abstract
[Objective]To identify hub genes closely associated with heat stress regulatory pathways and stress-resistance traits,and to provide new insights into the molecular mechanisms underlying heat tolerance in maize(Zea mays).[Method]A heat-tolerant inbred line,PHBA6,and a heat-sensitive inbred line,Ji63,were used as experimental materials.Plants were grown under both field and greenhouse conditions,and heat stress was imposed at the early flowering stage.Based on transcriptome sequencing,the molecular response characteristics of tassels from the two genotypes under heat stress were systematically compared.[Result]A total of 1 288 differentially expressed genes(DEGs)were identified in PHBA6,whereas 912 DEGs were detected in Ji63 under heat stress.Weighted gene co-expression network analysis(WGCNA)was further performed to construct gene co-expression networks associated with stress-resistance phenotypic traits,resulting in the identification of 15 gene modules significantly correlated with tassel heat tolerance.Among them,four modules showing the strongest correlations with target traits were selected,and a set of potential key genes was screened.These genes were mainly involved in biological processes such as transcriptional regulation,protein homeostasis maintenance,reactive oxygen species metabolism,and stress signal transduction.[Conclusion]This study systematically reveals the key regulatory network characteristics of maize tassels under heat stress and identifies a group of hub genes closely associated with heat tolerance and their potential regulatory pathways.关键词
玉米/热胁迫/加权基因共表达网络/转录组学Key words
maize/heat stress/weighted gene co-expression network/transcriptome引用本文复制引用
秦天元,吕玉平,阿不来提·阿布拉,韩登旭,董远,张雪,杨杰..基于WGCNA的玉米雄穗耐热相关共表达模块鉴定和核心基因发掘[J].生物技术通报,2026,42(10):55-66,12.基金项目
新疆维吾尔自治区"天池英才"项目,新疆维吾尔自治区农业科学院稳定支持(xjnkywdzc-2024003-13,xjnkywdzczc-2025001-43),新疆维吾尔自治区重大科技专项(2024A02003-2),新疆维吾尔自治区重点研发计划(2024B02008-1),新疆维吾尔自治区"天山英才"项目 （xjnkywdzc-2024003-13,xjnkywdzczc-2025001-43）
