生物技术通报2026，Vol.42Issue(10)：98-107,10.DOI:10.13560/j.cnki.biotech.bull.1985.2025-1388
藜麦G6PDH基因家族的鉴定及CqG6PDH1耐盐性分析
Identification of G6PDH Gene Family in Chenopodium quinoa and Functional Analysis of CqG6PDH1 in Salt Tolerance
摘要
Abstract
[Objective]Based on the information of genome and transcriptome,the members of the G6PDH gene family in quinoa were identified through bioinformatics methods.The function of the candidate gene G6PDH1 in salt tolerance was investigated.This study provides theoretical basis and gene resources for researching the salinity tolerance mechanisms in quinoa(Chenopodium quinoa Willd.).[Method]Based on the protein sequences of AtG6PDHs in Arabidopsis thaliana and OsG6PDHs in Oryza sativa,and the reported hidden markov model(HMM)files PF02781 and PF00479,the CqG6PDH gene family members were identified.Various bioinformatics tools such as TBtools,MEGA,MEME and WoLF PSORT were used to analyze the protein physicochemical properties,evolutionary relationships,gene structures,conserved motifs,chromosomal distribution,subcellular localization,and expression pattern of CqG6PDH gene family members.Additionally,RT-qPCR was used to analyze the expression pattern of CqG6PDH1 in different tissues and under salt stress,and A.thaliana was used to transform for validating the function of CqG6PDH1 in salt tolerance.[Result]Six CqG6PDH gene family members were identified,encoding proteins with 519-722 amino acids,with predicted molecular weights ranging from 59.30 to 79.58 kD and theoretical isoelectric points ranging from 5.24 to 8.69.CqG6PDHs were distributed on chromosomes 4,5,11,12,and 14,and it is predicted that their encoded proteins were localized in the cytoplasm and chloroplast.RNA-Seq data and RT-qPCR indicated that CqG6PDH1 responded to salt stress.The seeds and seedlings of transgenic Arabidopsis were treated with 175 and 250 mmol/L NaCl,respectively,the germination rate and the survival rate of Arabidopsis overexpressing CqG6PDH1 significantly increased,accompanied by low water loss rate and malondialdehyde(MDA)content,as well as enhanced SOD activity,POD activity and cell membrane stability.[Conclusion]Six CqG6PDH genes are identified in the quinoa and over-expressing CqG6PDH1 in Arabidopsis resulted in enhanced tolerance to salt.CqG6PDH1 is an important candidate gene for improving crop tolerance to salt and plays an important role in the regulatory process of responding to salt stress.关键词
藜麦/G6PDH基因家族/生物信息学分析/CqG6PDH1/耐盐性Key words
quinoa/G6PDH gene family/bioinformatics analysis/CqG6PDH1/salinity tolerance引用本文复制引用
王亦学,刘佳慧,许帆,胡鑫,王育川,侯丽媛,吴慎杰,郝曜山,董艳辉..藜麦G6PDH基因家族的鉴定及CqG6PDH1耐盐性分析[J].生物技术通报,2026,42(10):98-107,10.基金项目
山西省科技创新人才团队专项(202304051001040),山西省后稷实验室自主立项课题(202404010930003-Y20),山西农业大学生物育种工程(YZGC135),山西省育种联合攻关(NYGG28-02),山西农业大学科技创新提升工程(CXGC2025065) （202304051001040）
