中草药2026，Vol.57Issue(19)：7519-7531,13.DOI:10.7501/j.issn.0253-2670.2026.19.003
宽叶匙羹藤中C21甾体类成分及其促葡萄糖摄取活性研究
C21 steroidal components of Gymnema latifolium and their glucose uptake promoting activity
摘要
Abstract
Objective To investigate the C21 steroids from Gymnema latifolium and their glucose uptake-promoting activity.Methods The compounds were isolated and purified by various chromatographic techniques including silica gel,ODS,Sephadex LH-20 and semi-preparative HPLC,and their structures were elucidated on the basis of spectroscopic data.The glucose uptake-promoting activity of the isolates was evaluated using a HepG2 cellular glucose uptake model.Results A total of 18 C21 steroids were isolated and identified as gymlatifoside E(1),20-O-benzoyl-12-O-cinnamoyl-3β,5α,8β,12β,14β,17β,20-heptahydroxy-(20S)-pregn-6-ene(2),gymnepregoside H(3),prosapogenin(4),sylvepregoside E(5),gymnepregoside G(6),penupogenin(7),gymnepregoside I(8),cissasteroid A(9),cynsaccatol L(10),gymnemarsgenin(11),gnetumoside A(12),cynanchin F(13),gagamine(14),12-O-benzoylsarcostin(15),cissasteroid B(16),12,20-O-dibenzoylsarcostin(17),hemoside A(18).Among them,compound 1 is a new C21 steroid,and compounds 2-18 were isolated from G.latifolium for the first time.At non-cytotoxic concentrations,treatment with compounds 1-18 resulted in glucose uptake levels ranging from 0.70-to 1.73-fold of the control group in HepG2 cells,among which compounds 4,8-10,12-14,16,and 17 significantly promoted glucose uptake,reaching 1.38-to 1.73-fold of the control group.Conclusion G.latifolium is rich in C21 steroids,and some of these compounds exhibit promising potential anti-diabetic activity,warranting further investigation.关键词
宽叶匙羹藤/C21甾体/宽叶匙羹藤苷E/抗糖尿病活性/糖摄取Key words
Gymnema latifolium Wall.ex Wight/C21 steroidal glycosides/gymlatifoside E/anti-diabetic activity/glucose uptake分类
医药卫生引用本文复制引用
卢羽玲,刘美余,陈连,赵秋华,肖昔文,张进燕,卢汝梅..宽叶匙羹藤中C21甾体类成分及其促葡萄糖摄取活性研究[J].中草药,2026,57(19):7519-7531,13.基金项目
国家自然科学基金项目(82360823) （82360823）
广西自然科学基金项目(2025GXNSFBA069359) （2025GXNSFBA069359）
广西中医药大学博士启动基金(2023BS012) （2023BS012）
