中草药2026，Vol.57Issue(19)：7541-7547,7.DOI:10.7501/j.issn.0253-2670.2026.19.005
小黄构的化学成分及生物活性研究
Chemical constituents and bioactivities of Wikstroemia micrantha
摘要
Abstract
Objective To investigate the chemical constituents and biological activities of Wikstroemia micrantha(Thymelaeaceae).Methods The chemical constituents were isolated and purified via multiple chromatographic techniques including normal-phase silica gel column chromatography,ODS reversed-phase column chromatography and Sephadex LH-20 gel permeation chromatography.The structures of isolated compounds were elucidated by physicochemical properties,1D NMR,and ESI-MS spectroscopic analyses.The cytotoxic activities of all compounds against five human tumor cell lines(human breast cancer MCF-7 cells,human ovarian cancer SKOV3 cells,human colon cancer HCT-116 cells,human promyelocytic leukemia HL-60 cells,and human hepatocellular carcinoma HepG2 cells)were screened by the CCK-8 assay.Enzyme inhibition assays were performed to evaluate the inhibitory effects of the compounds on α-glucosidase,butyrylcholinesterase(BChE)and acetylcholinesterase(AChE).Results A total of ten compounds were isolated from the ethanol extract of the dried branches and leaves of W micrantha,and their structures were identified as syzalterin(1),2-(p-hydroxyphenoxy)-6,8-dimethyl-5,7-dihydroxychromone(2),genkwanin(3),luteolin(4),7-hydroxycoumarin(5),daphnoretin(6),daphnetin(7),2,2'-oxybis(1,4-di-tert-butylbenzene)(8),(+)-3-hhydroxy-1,5-diphenylpentan-1-one(9),1,5-diphenylpentan-1-one(10).In vitro antitumor activity screening revealed that compounds 2 and 4 exhibited weak cytotoxicity against various tumor cell lines,with their median inhibitory concentration(IC50)values ranging from 18 to 50 μmol/L.In α-glucosidase inhibitory assay,compounds 1,2 and 7 displayed moderate inhibitory activity,with IC50 values of(15.51±0.49),(15.99±1.15)and(19.65±1.71)μmol/L,respectively.Results from the in vitro BChE inhibitory assay indicated that compound 7 exerted potent inhibitory activity with an IC50 value of(5.63±0.20)μmol/L,whereas compound 9 exhibited moderate inhibitory activity[IC50=(13.22±0.84)μmol/L].None of the isolated compounds 1-10 showed notable inhibitory activity against AChE.Conclusion Compounds 1-10 were isolated from W micrantha for the first time.Bioactivity screening demonstrated that several isolates possessed favorable antitumor activity and selective BChE inhibitory effects.This study provides chemical material basis and experimental data support for further research and development of the medicinal material W.micrantha.关键词
荛花属/小黄构/抗肿瘤活性/酶体外抑制活性/芫花素/木犀草素/伞形花内酯/瑞香素Key words
Wikstroemia Endl./Wikstroemia micrantha Hemsl./antitumor activity/in vitro enzyme inhibitory activity/genkwanin/luteolin/7-hydroxycoumarin/daphnetin分类
医药卫生引用本文复制引用
侯泉杰,宋驰,沈云亨,蔡梅超,祖先鹏..小黄构的化学成分及生物活性研究[J].中草药,2026,57(19):7541-7547,7.基金项目
上海市卫生健康委员会中医药科研项目(2024QN057) （2024QN057）
海军军医大学"深蓝"工程启航人才计划 （）
海军军医大学教学成果立项培育项目(JPY2024B06) （JPY2024B06）
山东省中医药科技项目(M20251701) （M20251701）
山东中医药大学科学研究基金项目(KYZK2024M21) （KYZK2024M21）
