中草药2026，Vol.57Issue(19)：7573-7586,14.DOI:10.7501/j.issn.0253-2670.2026.19.008
铁皮石斛花外泌体样纳米颗粒的制备及体外抗肿瘤作用
Preparation and in vitro anti-tumor effects of extracellular vesicle-like nanoparticles from Dendrobium officinale flowers
摘要
Abstract
Objective To isolate and prepare extracellular vesicle-like nanoparticles from Tiepishihu(Dendrobium officinale)flowers(DF-EVLPs),and evaluate their specific targeting ability for colon cancer cells,as well as investigate their in vitro targeting and anti-tumor effects against colon cancer cells,in order to provide experimental evidence for the development of targeted delivery systems based on medicinal and edible plants.Methods DF-EVLPs were isolated fromD.officinale flowers and their morphology and particle size were characterized.The uptake of DF-EVLPs by CT26 colon cancer cells was observed using pKH26 fluorescence labeling.The effects of DF-EVLPs on the proliferation,migration and apoptosis of CT26 cells and their mechanism were evaluated using CCK-8 assay,scratch wound healing experiment,flow cytometry and Western blotting.Results The obtained DF-EVLPs showed a uniform cup-shaped morphology with an average particle size of(156.9±7.2)nm.The cell uptake experiment demonstrated that DF-EVLPs could be efficiently internalized by CT26 colon cancer cells,and the uptake efficiency of DF-EVLPs for colon cancer cells was significantly higher than that of normal colonic epithelial cells,indicating their good specific targeting ability.The in vitro functional experiments showed that DF-EVLPs inhibited the proliferation of CT26 cells in a concentration-dependent manner,significantly reduced cell migration ability,and induced cell apoptosis.Conclusion This study successfully prepared extracellular vesicle-like nanoparticles from D.officinale flowers.As a new type of food-derived nanotherapeutic agent,DF-EVLPs can specifically target colon cancer cells and exert in vitro anti-tumor activity.This study reveals a new biological activity function of D.officinale flowers and provides experimental evidence for dietary intervention for colorectal cancer based on the"medicinal and edible"concept.关键词
铁皮石斛花/外泌体样纳米颗粒/结肠癌/抗肿瘤作用/靶向递送系统Key words
Dendrobium officinale flower/extracellular vesicle-like nanoparticles/colon cancer/anti-tumor effect/targeted delivery system分类
医药卫生引用本文复制引用
肖吕浪,王江英,张芳瑕,余梦竹,周英,朱月,汪祖华..铁皮石斛花外泌体样纳米颗粒的制备及体外抗肿瘤作用[J].中草药,2026,57(19):7573-7586,14.基金项目
贵州省现代中药创制全省重点实验室(黔科合平台[2025]019) （黔科合平台[2025]019）
中药制剂与大健康产品开发贵州省科技创新领军人才工作站(黔科合平台KXJZ[2025]042) （黔科合平台KXJZ[2025]042）
贵州中医药大学纳米药物技术研究中心(贵中医ZX合字[2024]070) （贵中医ZX合字[2024]070）
