茶叶科学2001，Vol.21Issue(1)：21-25,5.
茶树S-腺苷甲硫氨酸合成酶 基因的克隆和序列分析
Cloning and Sequencing of S-adenosylmethionine Synthetase Gene in Tea Plant
摘要
Abstract
Total RNA was extracted from tea leaf and the first strand ofcDNA was synthesized with reverse transcription.The principal fragment，3'-end fragment and 5'-end fragment of S-adenosylmethionine synthetase gene (SAM gene)was amplified with the first strand cDNA as template and three pairs primers by PCR. Complete SAM gene sequence was obtained by BLAST comparison of the three fragments and splicing according to the overlapping regions. The sequence of the SAM gene is 1303 base pairs (bp)in length with an opening reading frame （ORF） encoding 394 amino acids. The cDNA sequence shows a significant homology to the SAM genes from Actinidia chinensis (87%)，Lycopersicon esculentum (83%)，Catharanthus roseus (83%). The amino acid sequences shares 90%-92% identity with SAM synthetase from Populus trichocarpa，Actinidia chinensis，Petunia hybrida，Catharanthus roseus, etc. Based on sequence homology，it was suggested that the obtained cDNA was a SAM gene in tea plant. The isolation of the SAM gene established a good foundation for further study on stress physiology，senescence physiology and caffeine biosynthesis in tea plant.关键词
茶树/SAM合成酶/基因克隆/RT-PCR分类
农业科技引用本文复制引用
冯艳飞,梁月荣..茶树S-腺苷甲硫氨酸合成酶 基因的克隆和序列分析[J].茶叶科学,2001,21(1):21-25,5.基金项目
中-日国际合作项目"C0056"研究内容之一。 （）
