茶叶科学2009，Vol.29Issue(4)：251-256,6.
EGCG与锌离子互作对PC-3细胞能量代谢的影响
Effect of Interactions Between EGCG and Zn2+ on Energy Metabolism of PC-3 Cells
孙世利 1凌彩金 1于海宁 2沈生荣 3苗爱清 1潘顺顺 1庞式 1卓敏 1赵超艺1
作者信息
- 1. 广东省农业科学院茶叶研究所,广东,广州,510640
- 2. 浙江工业大学药学院,浙江,杭州,310032
- 3. 浙江大学生物系统工程与食品科学学院,浙江,杭州,310029
- 折叠
摘要
Abstract
In the present work,the effect of treatments with EGCG,Zn2+ and EGCG+Zn2+ on inducing apoptosis and content of adenylate nucleotide (ATP,ADP and AMP) in PC-3 cells was investigated by chromatin staining with Hoechst 33258 and HPLC.Hoechst 33258 staining revealed the appearance of condensed chromatin and fragmented apoptotic nuclei under fluorescence microscopy at a concentration of 80 μmol/L EGCG,80 μmol/L Zn2+ and 80 μmol/L EGCG+80 μmol/L Zn2+ in PC-3 cells.In the determination of adenylate nucleotide,after treated with EGCG,Zn2+ and EGCG+Zn2+,content of total adenylate nucleotide,EC and ATP/ADP value were significantly decreased in PC-3 cells,which demonstrated that EGCG and Zn2+ have a markedly inhibitory effect on energy metabolism of PC-3 cells.关键词
EGCG/锌离子/PC-3细胞/细胞凋亡/腺苷酸/能量代谢Key words
EGCG,Zn2+,PC-3 cells,apoptosis,adenosine triphosphate,energy metabolism分类
农业科技引用本文复制引用
孙世利,凌彩金,于海宁,沈生荣,苗爱清,潘顺顺,庞式,卓敏,赵超艺..EGCG与锌离子互作对PC-3细胞能量代谢的影响[J].茶叶科学,2009,29(4):251-256,6.
