食品与发酵工业2001，Vol.27Issue(4)：25-29,5.
高产柠檬酸的SHAM特性黑曲霉突变株的选育
High Producing Citric Acid by Selection of SHAM Mutants of Aspergillus niger
王庆昭 1李剑 1刘伟 1杨枫 1高年发1
作者信息
摘要
Abstract
The influences of SHAM (salicylhydroxamic acid) on the respiratory system of Aspergillus niger were studied. The 5mg/60mL SHAM will almost completely inhibit the citric acid production in shaken cultures. When W3 was cultivated on agar plate (L1), which contains 10mg/10mL SHAM, it only can produce 10% spores. When it is cultivated on plate (L2),which contains 20mg/10mL SHAM, it can not produce spores. According to this, many SHAM mutants which can produce normal amount of spores on L1 plate and many SHAM mutants which can produce spores on L2 plate were selected. By means of fermentation test SHAM-2 strain can produce 40% higher of citric acid than original strain W3 and the yield of citric acid is 91.7%.关键词
黑曲霉柠檬酸60Co-γ诱变全玉米SHAM敏感株分类
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王庆昭,李剑,刘伟,杨枫,高年发..高产柠檬酸的SHAM特性黑曲霉突变株的选育[J].食品与发酵工业,2001,27(4):25-29,5.
