食品科学2008，Vol.29Issue(6)：292-297,6.
微乳液介质中巯基葡聚糖凝胶分离富集催化动力学光度法测定痕量硒(IV)
Catalytic Kinetic Spectrophotometric Determination of Trace Selenium(IV)in Microemulsion after Separation and Enrichment by SDG
摘要
Abstract
Acatalytic spectrophotometry for the determination of trace selenium(IV)based on its catalytic effect on discoloring reaction of oxidation of with potassium brornate in weak acid solution and in the presence of microcmulsion medium is described.The above reaction is followed spectrophotometrically by measuring the decrease in the absorbance at 450 mn for the catalytic reaction of 5-NO2-PADAP.Under optimum conditions,the calibration graph is linear in the range of 0.0～1.0 μg/L of Se(Ⅳ)at 450 nm.The detection limit achieved is 0.013μ g/L.Combined with sulphydryl dextrane gel(SDG)separation and enrichment,the method has been successfully applied to the determination of Se(IV)in foodstuff and human hair samples with the relative standard deviation of 2.9%～4.3%andthe recovery of 96.3%～103.8%.the results are in good agreement with those provided by ICP-AES method.关键词
催化动力学光度法/硒(IV)/2-(5.硝苯2.吡啶偶氮)-5-二已氨基酚(5-NO2-PADAP)/微乳液/分离/富集Key words
catalytiv kinetic spectrophotometry/selenium(IV)/2-(5-nitro-2-pyridylazo)-5-diethylaminophenyl(5-NO2-PADAP)/microemulsion medium/separation:enrichment分类
轻工纺织引用本文复制引用
周之荣,王群,章淑媛,张纪文,张圆玲,苏志文..微乳液介质中巯基葡聚糖凝胶分离富集催化动力学光度法测定痕量硒(IV)[J].食品科学,2008,29(6):292-297,6.基金项目
广东药学院科研基金资助项目(2007GGW03) （2007GGW03）
