食品工业科技Issue(2):30-32,3.
棉织物上SESA活化法固定化脂肪酶工艺的研究*
Study on immobilization of lipase onto cotton fiber activated by SESA
彭立凤 1刘新喜2
作者信息
- 1. 河北师范大学化学系,
- 2. 河北师范大学学报编辑部,
- 折叠
摘要
Abstract
The conditions of the lipase immobilization onto cotton fiber activated by p-β-sulfuric acid ester ethyl sulfone aniline (SESA) have been studied. The optimal temperature and pH of the immobilized lipase were investigated, and the operating stability of the immobilized lipase was studied too. The results showed that the optimal conditions for the immobilization were that the pH of the reaction for the cotton fiber with SESA was 10.0,the linking pH of lipase to fiber was 7.0,the linking time was 12h,and the concentration of lipase was 6mg· mL- 1 .Then 35 U· g(fiber) - 1 · min- 1 activity of immobilized lipase was obtained. The optimal temperature and pH of the immobilized lipase were 35 ℃ and 8.0 respectively, and its half life was 6 days .关键词
脂肪酶固定化棉织物对-β-硫酸酯乙砜基苯胺(SESA)分类
轻工纺织引用本文复制引用
彭立凤,刘新喜..棉织物上SESA活化法固定化脂肪酶工艺的研究*[J].食品工业科技,2001,(2):30-32,3.