茶叶科学2001，Vol.21Issue(1)：60-64,5.
茶多酚中儿茶素类的HPLC分析方法学考察
Study on the Method of HPLC Analysis of Catechins in Tea Polyphenols
摘要
Abstract
A kind of analytical method by high performance liquidchromatography( HPLC) for tea catechins with ingradient elution system was put forward. In the meantime，the method was investigated in the methodological aspect through separa ting and determining the main four catechins (EC，ECG，EGC，EGCG) of tea polyphe nols. The optimum chromatographic conditions were as follows：ODS column (5 μm ， 4.6×250 mm)，water/acetonitrile/ethylacetate (v/v/v，8 6∶12∶2) as mobile phase ( add H2SO4 to adjust pH 3.0～4.0)，UV detector (280 nm)，flow rate:1.0 ml/ min. Moreover，the precision and stability of the method was discussed.关键词
儿茶素/HPLC/方法学分类
化学化工引用本文复制引用
于海宁,沈生荣,臧荣春,冯萍..茶多酚中儿茶素类的HPLC分析方法学考察[J].茶叶科学,2001,21(1):60-64,5.基金项目
浙江省科委大型仪器分析测试基金资助项目(编号：99129) （编号：99129）
