食品科学2006，Vol.27Issue(12)：62-66,5.
响应曲面法研究磁场对超氧化物歧化酶活性的影响
Effect of Magnetic Fields on Superoxide Dismutase Activity Using Response Surface Methodology
摘要
Abstract
The effect of three variables (temperature, magnetic intensity, and exposure time) on the activity of superoxide dismutase (EC 1.15.1.1) isolated from Saccharomyces cerevisiae was studied by Response Surface Methodology (RSM). This multivariate methodology offers an empirical approach to the study of enzyme assays and allows to detect the interaction between different variables of the system. Analysis of variance (ANOVA) showed that a second order polynomial model (SOP)generated response surface and contour plots, and the model may predict the experimental data. Lack-of-fit tests did not result in a significant F-value. Determination coefficients (R2) were greater than 94%. The optimum treating parameters were obtained as: temperature, 15.5 ℃; magnetic intensity, 0.95T; time of magnetic field exposure, 64.5 minutes.关键词
超氧化物歧化酶/酿酒酵母/响应曲面法Key words
superoxide dismutase/Saccharomyces cerevisiae/response surface methodology分类
轻工纺织引用本文复制引用
王山杉,李冰,李琳..响应曲面法研究磁场对超氧化物歧化酶活性的影响[J].食品科学,2006,27(12):62-66,5.基金项目
国家自然科学基金重点项目(20436020) （20436020）
国家自然科学基金青年基金项目(20306007) （20306007）
广东省自然科学基金重点项目(04105934) （04105934）
