食品科学2001，Vol.22Issue(2)：64-67,4.
高效液相色谱法测定何首乌中大黄酸、大黄素、大黄素甲醚的含量
Determination of Emodin Acid Emodin and emodin Methyl-ether in Polygonum multiforum by HPLC
赵仁邦 1葛微 1崔同 1刘淑晨 1张冬1
作者信息
摘要
Abstract
Using 70% methanol, the Polygonum multiforum extract wasconcentrated by ultrasonic method .Then concentrated extract was leached with chloroform and separated into free and bound anthraquinones. The mobile phase was V(CH3OH):V(C3H7OH):V(H2O)=80:10:10 (control pH to 3.0 with phosphorus acid ).The HPLC system was consisted of Hypersil BDS C 18(5 μm,250nm × 46nm) and UV detector set at 439nm.The experiment results show that the average recovery of Emodin was 100.7% (RSD=1.77),and that of Emodin methylether was 98.7% (RSD=1.77%) .The relative standard deviation for Emodin acid ,Emodin and Emodin methylether were 1.18%,0.79% and 0.75% respectively.关键词
高效液相色谱何首乌蒽醌类大黄酸大黄素大黄素甲醚Key words
HPLC Polygonum multiforum Anthraquinones Emodin acid EmodinEmodin methylether分类
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赵仁邦,葛微,崔同,刘淑晨,张冬..高效液相色谱法测定何首乌中大黄酸、大黄素、大黄素甲醚的含量[J].食品科学,2001,22(2):64-67,4.
