现代食品科技2008，Vol.24Issue(8)：787-790,4.
响应曲面法对壳聚糖亚铁离子配合物酶解的优化
Optimization of Enzymolysis of Chitosan-Fe2+ by Response Surface Methodology
摘要
Abstract
A new method for controllable preparation of chitosan oligosaccharides was developed by enzymolyzing the coordination complex of chitosan and Fe2+ (Chitosan-Fe2+,CTS-Fe2+) with papain in this study. The effects of four process parameters including pH, temperature, substrate concentrations and enzyme-substrate ratio on the enzymolysis of CTS-Fe2+ with papain were investigated by using the response surface methodology (RSM). To reveal the viscosity decrease percentage (VDP) of the enzymolyzed CTS-Fe2+, a second order polynomial mathematic model of the reaction was built according to the analysis results. The optimum pH value, temperature, substrate concentration and enzyme-substrate ratio were 4.46, 44.95 ℃, 1.97％ and 1.75％, respectively.关键词
木瓜蛋白酶/壳聚糖亚铁离子配合物/响应曲面法/最佳工艺参数Key words
papain/Chitosan-Fe2+/response surface methodology/optimum process parameters分类
轻工纺织引用本文复制引用
蒋寅,王兆梅,宋国胜,郭桦,郭祀远..响应曲面法对壳聚糖亚铁离子配合物酶解的优化[J].现代食品科技,2008,24(8):787-790,4.基金项目
Supported by Item of Special Funds for Scientific Research on DoctorSubject in Colleges to Ministry of Education (20050561014)Reference （20050561014）
