食品与机械2009，Vol.25Issue(4)：95-99,5.
荧光光谱分析法用于维生素药片中核黄素的检测
Investigation of fluorescence spectrometry for determination of riboflavin in vitamin tablets
摘要
Abstract
A fluorescence spectrometry for detection of pharmaceuticals like riboflavin in vitamin B2 (VB2) in Tris-HCl buffer of pH=7. 00 with excitation/emission wavelength of 467/523.5nm has been developed in this paper. The detection limit for riboflavin could be e-valuated to be a supra-low concentration of 15.7 pg/mL, which was better than that of reported data. The presented method possessed high sensitivity, nice reproducibility and stability, and no obvious in-terferences from Na+, K+, Ba2+, Mg2+, Zn2+, vitamin C. B1, B6, oryzanol and pyrazine compounds were observed. With a recovery rate of 96.53% to 103.94%, the method has been applied to determination of amount of VB2 in tablets which was in well agreement with that of UV/Vis spectrophotometry, indicating that it can be used for the detection of practical therapeutic drugs.关键词
荧光光谱分析法/药物分析/核黄素/痕量测定/氢键作用Key words
Fluorescence spectrometry/Pharmaeeutical analysis/Riboflavin/Trace determination/Hydrogen-bond interaction分类
轻工纺织引用本文复制引用
曹忠,龙姝,卢菲娜,吕品,黄敏,丁飞,熊波,赵永福,池梅丽..荧光光谱分析法用于维生素药片中核黄素的检测[J].食品与机械,2009,25(4):95-99,5.基金项目
国家自然科学基金项目(编号:20775010) （编号:20775010）
湖南省科技厅科技计划项目(编号:2008FJ3014) （编号:2008FJ3014）
湖南省自然科学基金项目(编号:09JJ3016) （编号:09JJ3016）
湖南省教育厅科学研究基金项目(编号:08B004,07A006,06C098) （编号:08B004,07A006,06C098）
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