食品与机械2011，Vol.27Issue(1)：7-10,4.DOI:10.3969/j.issn.1003-5788.2011.01.002
改性磁性壳聚糖微球固定化乳糖酶
Immobilization of lactase on modified magnetic chitosan microspheres
摘要
Abstract
Modified magnetic chitosan microspheres (MMCM) were prepared with reversed-phase suspension polymerization method using HEMA and GMA as monomers and ammonium persulfate as initiator. Lactase was immobilized on MMCM via adsorption, covalent bonding and cross-linking with glutaraldehyde. The optimum conditions of lactase immobilization were: MMCM were previously swelled in 0. 1 M pH 7.0 phosphate buffer sufficiently, lactase was added to 2.0 U per mg of carrier and incubated at 4 ℃ for 3 h, reacted with 0. 1％ glutaraldehyde for 4 h. The activity of immobilized lactase was 685.14 U/g and the activity recovery was 34. 3％. In comparison with the free enzyme , the pH and thermal stability of immobilized lactase were increased. After continuous operation for 10 cycles, the immobilized lactase activity was remained over 70％.关键词
磁性壳聚糖微球/接枝改性/固定化/乳糖酶引用本文复制引用
钱婷婷,杨瑞金,华霄,赵伟,张文斌,金征宇..改性磁性壳聚糖微球固定化乳糖酶[J].食品与机械,2011,27(1):7-10,4.基金项目
江苏省自然科学基金创新学者攀登项目(编号:BK2008003) （编号:BK2008003）
食品科学与技术国家重点实验室目标导向项目(编号:SKLF-MB-200804) （编号:SKLF-MB-200804）
自由探索项目(编号:SKLF-TS-200903) （编号:SKLF-TS-200903）
无锡市科技计划项目(编号:CLE00902) （编号:CLE00902）
