食品与机械2011，Vol.27Issue(1)：22-25,4.DOI:10.3969/j.issn.1003-5788.2011.01.006
AB-8大孔树脂分离纯化北五味子藤茎总木脂素的研究
Study on separation and purification of total lignans from caculis of Schisandra Chinensis(Turcz.)Baill by AB-8 macroporous resin
摘要
Abstract
According to the UV spectrophotometry method for measuring the total lignans content from caulis of Schisandra chinensis,the author screens out the best resin for obtaining pure lignans by calculating the adsorption and desorption rate of six types of macro porous resins. With the evaluation of the dosage of loading buffer,velocity of adsorption, the volume of distilled water and alcohol in the eluent, the writer then comes out with the best purification process for obtaining total lignans content from AB-8 macro porous resins. As the experiment reveals, in order to obtain lignin with the density of 1.511 mg/mL, the maximum loading amount is approximately 6 times to the column volume as well as distilled water and 95％ alcohol takes 8 and 5 the column volume, respectively. Also,the flow rate of elution is measured as 1 mL/min. In conclusion,with this advanced purification process, the elution rate can reach as high as 75. 53％, providing us with a better way to purify lignans content from caulis of Schisandra chinensis and will be make good use in the industrial world in the future.关键词
北五味子/藤茎/木脂素/大孔树脂引用本文复制引用
薛雪,孟宪军,李斌,李元甦..AB-8大孔树脂分离纯化北五味子藤茎总木脂素的研究[J].食品与机械,2011,27(1):22-25,4.基金项目
"十一五"国家科技支撑计划项目(编号:2006BAI09B04) （编号:2006BAI09B04）
