食品与发酵工业2011，Vol.37Issue(1)：21-25,5.
乳源血管紧张素转移酶抑制肽在乳酸乳球菌中的表达
Expression Milk-derived Angiotensin-I Converting Enzyme Inhibitory Peptides in Lactococcus lactis
摘要
Abstract
Objective:To express Milk-derived angiotensin-Ⅰ converting enzyme (ACE) inhibitory peptides in Lactococcus lactis (L.lactis).Methods:The four different Bovine casein-derived peptides were chosen and designed as tandem peptides (TP) with digestive enzyme restriction site among them to ensure the potent ACE inhibitory activity of TP could be activated by pepsin and trypsin in human digestive juice.The TP gene was synthesized artificially based on its amino acid sequence and bias codon of L.lactis.Then, the TP gene was co-expressed with green fluorescent protein (GFP), which was cloned in expression vector pSEC-E7 resulting in the pSEC-TP:GFP plasmid.This recombinant plasmid was transformed into L.lactis strain NZ9000 carrying regulatory genes nisR and nisK to obtain the strain NZ9000 (pSEC-TP:GFP) by electroporation.The expression of TP:GFP gene induced by Nisin was identified by RT-PCR, SDS-PAGE and Confocal scanning laser micrographs.Results: RT-PCR showed that the TP: GFP was expressed at the RNA level.SDS-PAGE analysis showed target proteins product of 35 ku.Conclusion:The expression of Milk-derived ACE inhibitory peptide has been detected in Lactococcus lactis.关键词
乳源血管紧张素转移酶抑制肽/乳酸乳球菌/表达引用本文复制引用
曲行光,满朝新,姚丽燕,赵凤,杨士芹,姜毓君..乳源血管紧张素转移酶抑制肽在乳酸乳球菌中的表达[J].食品与发酵工业,2011,37(1):21-25,5.基金项目
国家863项目(2008AA10Z311) （2008AA10Z311）
国家科技支撑计划项目(2009BADB9B06) （2009BADB9B06）
黑龙江省青年科学基金项目(QC2009C55) （QC2009C55）
东北农业大学博士启动基金(2009RC07) （2009RC07）
