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酶法制取橙皮果胶的研究

韩冬梅 廖小军 李淑燕 胡小松 吴继红

食品工业科技2011,Vol.32Issue(2):183-186,4.
食品工业科技2011,Vol.32Issue(2):183-186,4.

酶法制取橙皮果胶的研究

Cellulase extraction of pectin from orange peel

韩冬梅 1廖小军 1李淑燕 1胡小松 1吴继红1

作者信息

  • 1. 中国农业大学食品科学与营养工程学院,农业部果蔬加工重点开放实验室,果蔬加工教育部工程研究中心,北京100083
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摘要

Abstract

The characteristics of cellulase system is complicated,cellulase from different sources act with different effects in pectin extraction.The extraction of pectin from orange peel by cellulases which was different from the ones in literatures was studied.One kind of Iow-cost cellulase suitable for pectin extraction was screened out from three kinds of cellulases.And the effects of extraction pH, temperature,time, enzyme concentration on the pectin yield were studied by quadratic regression rotational composite design.The best effective factors were determined.Via statistical software of Design Expert analysising, the model was significant and the lack of fit was not significant with obvious statistical significance.The optimal combination forecasting model was drew on volume of enzyme 3.38mL, time 6.66h, temperature 47.5℃, pH4.95, pectin yield of forecasting model was 14.43% and verification experiment was 14.79%.Pectin yield was improved greatly and much higher than the reported in literatures,and cellulose was Iow-cost,so cost of production can be saved greatly.

关键词

橙皮/果胶/纤维素酶/二次通用旋转组合设计

分类

轻工纺织

引用本文复制引用

韩冬梅,廖小军,李淑燕,胡小松,吴继红..酶法制取橙皮果胶的研究[J].食品工业科技,2011,32(2):183-186,4.

基金项目

国家863计划(2007AA10Z307). (2007AA10Z307)

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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