食品工业科技2011，Vol.32Issue(2)：178-180,194,4.
乳酸杆菌脉冲场凝胶电泳分子分型的条件优化
Optimization in molecular typing of Lactobacillus by pulsed field gel electrophoresis
摘要
Abstract
Objective:A method of molecular typing of Lactobacillus was established and optimized by pulsed field gel electrophoresis( PFGE).Methods:PFGE was used in the genetic typing study of Lactobacillus to optimize the factors influencing the typing of PFGE including different restriction endonuclease, different reaction concentration and reaction time. With these optimized factors, typing research was conducted to the 22 reference strains of Lactobacillus. Results : Restriction endonucleases Ascl was selected in this study with the optimized condition to be reaction concentration of 30U/150μL, reaction time of 4.5 hours and pulse time of 1 ～ 15s. By this technique, five electrophoretypes were distinguished in five reference strains of the different species, but the Lactobacillus within a species could not be distinguished by the PFGE. Conclusion: PFGE could be used to classify and identify Lactobacillus.关键词
脉冲场凝胶电泳(PFGE)/乳酸杆菌/分子分型分类
轻工纺织引用本文复制引用
许飞利,杨晓光,郭云昌,付萍,王洪新,李志刚,刘秀梅..乳酸杆菌脉冲场凝胶电泳分子分型的条件优化[J].食品工业科技,2011,32(2):178-180,194,4.基金项目
转基因生物新品种培育重大专项(2008ZX08011-005). （2008ZX08011-005）
