茶叶科学2011，Vol.31Issue(1)：33-39,7.
茶树多酚氧化酶基因的生物信息学分析及原核表达
Bioinformatic Analysis and Prokaryotic Expression of Polyphenol Oxidase Gene in Tea Plant (Camellia sinensis)
摘要
Abstract
The polyphenol oxidase (PPO, GenBank accession No. DQ812086) gene was amplified by PCR.Bioinformatics analysis indicated that the cds of PPO DNA sequence is 1800 bp, which encoded a protein of 599 amino acid residues (molecular formula: C3008H4677N813O900S18) and predicted that it had two Cu-band functional domains with no transmembrane. The phylogeny analysis showed that it has a most close phylogenetic relationship with Camellia nitidissima. The PPO was cloned into pET28a vector to construct recombination prokaryotic expression vector pET-ppo. After transformed to Elcoli BL21 and induced by isopropyl-β-D-thiogalactopyranoside(IPTG), recombinant protein about 71 kD was expressed in pET28a system and separated by SDS-PAGE electrophoresis.关键词
多酚氧化酶/生物信息学分析/原核表达Key words
polyphenol oxidase/ bioinformatics analysis/ prokaryotic expression分类
农业科技引用本文复制引用
王乃栋,张丽霞,向勤锃,李巍巍..茶树多酚氧化酶基因的生物信息学分析及原核表达[J].茶叶科学,2011,31(1):33-39,7.基金项目
山东省自然科学基金项目(ZR2010CM026)、山东省泰安市大学生创新行动计划项目(2009D1016) （ZR2010CM026）
