茶叶科学2011，Vol.31Issue(1)：59-65,7.
利用基因芯片筛选茶树芽叶紫化相关基因
Young Shoot Purple-Related Gene Screening in Tea Plant (Camellia sinensis) by Using cDNA Microarray
摘要
Abstract
Young shoots purple-related differentially expressed genes of tea plant have been found in purple and green shoots line of Longjing Qunti by cDNA microarray technique. There were 43 differentially expressed genes relating to purple shoots, among them 17 were up-regulated and 26 were down-regulated genes. Three differentially expressed genes and two no change genes were selected to verify the results of cDNA microarray hybridizations. The result of real time RT-PCR analysis was consistent with that of cDNA microarray. Then all of the differentially expressed genes were annotated functionally by Blast X search against the NCBI non-redundant protein databases.The molecular function of differentially expressed genes mainly included energy metabolism, secondary metabolism and transcriptional regulation. Especially two genes and two transcription factors involved in the biosynthesis pathway of anthocyanidin were selected from the results of cDNA microarray hybridizations. The phenylalanine ammonia lyase gene, MYB protein, WD40-like protein, and anthocyanidin reductase gene showed down-regulated 3.44, 2.25, 2.54 folds and up-regulated 2.09 folds, respectively. This study establishes a basis for further understanding the purple mechanism of tea young shoots and related gene cloning.关键词
茶树/基因芯片/紫芽/差异表达基因Key words
tea plant (Camellia sinensis)/ cDNA microarray/ purple shoots/ differentially expressed genes分类
农业科技引用本文复制引用
马春雷,姚明哲,王新超,金基强,陈亮..利用基因芯片筛选茶树芽叶紫化相关基因[J].茶叶科学,2011,31(1):59-65,7.基金项目
国家863计划(2006AA10Z171)、现代农业产业技术体系建设专项资金(Y3100291)、浙江省自然科学基金(Y3090041) （2006AA10Z171）
