摘要
Abstract
Pre-cooked beef liquid to centrifugation and filtration etc,was concentrated to the original volume of its 75％,50％, mainly determination of total nitrogen, amino nitrogen content. Staphylococcus aureus, E. coli, Salmonella strains for quality control, testing of three different concentrations of pre-cooked beef broth liquid alternative to ordinary sensitivity,and to observe the three kinds of quality control strains were pre-cooked beef at different concentrations of nutrient agar solution prepared on the growth of colony morphology. The results showed that:the original pre-cooked beef liquid ,concentrated to the original volume of 75％ ,50％ concentrated liquid TN contents were 2.044, 3.066, 4.088mg/mL, amino acid nitrogen contents were 0.945, 1.425, 1.890mg/mL. Phosphate test,alkaline precipitation test, pigment-producing testing and sensitivity met the media requirements. Three kinds of quality control strains of colony morphology, colony size, pigment-producing strains were in line with the growth characteristics. Concentrated solution in 50％ of the pre-cooked beef liquid nutrient broth prepared to cultivate 24h sensitivity can reach 10-9,the sensitivity of the strongest. Therefore TN,amino nitrogen content were up to 4.088,1.890mg/mL can be alternative to the concentrated solution of three quality control strains used for this culture of beef extract.关键词
牛肉预煮液/总氮/培养基/牛肉浸液分类
轻工纺织
