食品工业科技Issue(1)：287-289,3.
超高效液相色谱-质谱联用法快速测定豆酱中PC、LPC和GPC的含量
Rapid determination of PC,LPC and GPC in soybean paste by ultra performance liquid chromatography-mass spectrometry
摘要
Abstract
UPLC-MS was established to determine the content of PC, LPC and GPC in soybean paste. After pretreatment,the extract of soybean paste was injected directly into the system of UPLC.The analysis was carried out on ACQUITY UPLC BEH HILIC column(2.1 × 100mm, 1.7μm), using acetonitrile and ammonium acetate as the mobile phase for quantification. PC, LPC and GPC was identified by electrospray ionization mass spectrometry (ESI-MS) ,and electrospray was operated in positive ion mode to generate protonated ions. The ion at m/z782,m/z520 and m/z258 was recorded in the selective ionization recording (SIR). Good linearity between response ( peak area) and concentration was found over a concentration range of 0.191～1.910μg/mL for PC,0.236～2.360μg/mL for LPC,and 0.016～0.160μg/mL for GPC, the correlation coefficient of the calibration curve was 0.9959 for PC,0.9986 for LPC, and 0.9991 for GPC.The detection limit was 0.05μg/mL for PC,0.07μg/mL for LPC, and 0.005μg/mL for GPC. The quantification limit was 0.191μg/mL for PC,0.241μg/mL for LPC,and 0.0161μg/mL for GPC. The average recovery rates were 93.2％～97.8％ ,and the relative standard deviations were 1.5％～3.5％.This method is accurate,sensitive,short,and the sample treatment is simple,which can be used as a rapid determination of PC, LPC and GPC in soybean paste.关键词
PC/LPC/GPC/豆酱/超高效液相色谱/质谱分类
轻工纺织引用本文复制引用
成琳,陶冠军,王淼,陈卫,赵建新..超高效液相色谱-质谱联用法快速测定豆酱中PC、LPC和GPC的含量[J].食品工业科技,2011,(1):287-289,3.基金项目
十一五国家科技支撑计划(2009BADB9B05). （2009BADB9B05）
