食品工业科技Issue(1)：243-245,308,4.
AB-8树脂纯化柿叶总黄酮的工艺研究
Separation and purification process of total flavonoids from persimmon leaf with AB-8 resin
马燕燕 1鲁晓翔1
作者信息
- 1. 天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津,300134
- 折叠
摘要
Abstract
Objective:To choose the optimum resin for separation and purification of persimmon leaf flavonoids and analyze influenceable factors of separation and purification process. Methods:Three macroporous resins including AB-8,ADS-17and D3520 were used for separation of total flavonoids from persimmon leaf. During the adsorption and desorption process, with UV to assay total flavonoids,the different macroporous resins and their capacities were studied. Results:AB-8 resin possesses had much higher adsorption and desorption capacity. The optimum conditions were:the pH value of sample 6, current velocity 2BV/h, concentration of sample 3mg/mL,70％ alcohol as eluant for desorption, current velocity 2BV/h and eluant volume 3BV. An enriched total flavonoids extract with 34％ purity can be separated from crude persimmon leaf extract( about 9％ purity)in this way. Conclusion: It is feasible to separate and purify persimmon leaf flavonoids with AB-8 resin.关键词
柿叶/总黄酮/大孔吸附树脂/分离纯化分类
轻工纺织引用本文复制引用
马燕燕,鲁晓翔..AB-8树脂纯化柿叶总黄酮的工艺研究[J].食品工业科技,2011,(1):243-245,308,4.
