现代食品科技2011，Vol.27Issue(1)：6-10,25,6.
梨采后黑斑病拮抗菌J18的鉴定及防治效果的初步研究
Identification of a Bacterial Strain Bacillus subtilis J18 and Its Resistance to Pear Alternaria Rot Disease
宋聪 1宋水山 1关军锋2
作者信息
- 1. 河北省科学院生物研究所,河北石家庄,050051
- 2. 河北省农林科学院遗传生理所,河北石家庄,050051
- 折叠
摘要
Abstract
Abacterial strain with a high ability of inhibiting alternaria rot disease on pear was isolated from the pear fruits and orchard soil collected from different area across China with in vitro and in vivo methods. By morphological observation, physiological and biochemical characterization and the analysis of the 16S rDNA sequence, this strain was identified as Bacillus subtilis J18. Pretreatment with 108 CFU/mL cell suspension of B. subtilis J18 for 30s could decrease the incidence of alternaria rot on pear to 16.6％ after inoculation with pathogenic fungi Alternaria alternate (Fr.) Keissler. Supplement with salicylic acid, CaCl2 and chitosan resulted in a significant increase in biocontrol effect of B. subtilis J18. It also showed that the biocontrol effect of B. subtilis J18 on pear altemaria rot could reach up to 72.81％ and 77.07％ when combined with 2％ CaCl2 and 1％ chitosan, respectively. These results revealed apotential application B. subtilis J18 as an antagonist for controlling the fruit postharvest diseases.关键词
采后病害/拮抗菌/生物防治引用本文复制引用
宋聪,宋水山,关军锋..梨采后黑斑病拮抗菌J18的鉴定及防治效果的初步研究[J].现代食品科技,2011,27(1):6-10,25,6.
