食品与发酵工业2011，Vol.37Issue(4)：17-20,4.
双有机溶剂中黑曲霉脂肪酶催化阿魏酸的酯化反应
Esterification of Ferulic Acid by Aspergillus niger Lipase Catalysis in Binary Organic Solvents System
摘要
Abstract
In binary organic solvents system, the esterification between ferulic acid and oleyl alchol was investigated, catalyzed by the native Aspergillus niger lipase.The effective factors, such as lipase concentration, temperature and the molar ratio of ferulic acid to oleyl alchol on the esterification were studied.The results indicated that in the isooctane/2-butanone solvents system, under the reaction conditions of Aspergillus niger lipase concentration 0.2 g/mL, temperature 60 ℃, the molar ratio of ferulic acid to oleyl alchol 1∶ 8, substrate concentration of ferulic acid 0.39 mg/mL, and oleyl alcohol 4.3 mg/mL,the conversion rate of esterification was 97.6％.Under the optimal condition in the cyclohexane/2-butanone solvents system which was: Aspergillus niger lipase concentration 0.25 g/mL, temperature 60 ℃, the molar ratio of ferulic acid to oleyl alchol 1∶ 8, the substrate concentrations of ferulic acid at 0.49 mg/mL and oleyl alcohol at 5.37 mg/mL, the conversion rate of esterification was 91.0％.关键词
脂肪酶/酯化/有机溶剂/阿魏酸/油醇Key words
lipase/ esterification/ organic solvent/ ferulic acid/ oleyl alcohol引用本文复制引用
刘焕珍,王明兹,郭英佳,郑丽妃,Guo Zheng,Xu Xue-bing,陈必链..双有机溶剂中黑曲霉脂肪酶催化阿魏酸的酯化反应[J].食品与发酵工业,2011,37(4):17-20,4.基金项目
福建省科技厅重点项目(2007I0039,2008I0047),教育部留学回国人员科研启动基金资助课题 （2007I0039,2008I0047）
