食品与发酵工业2011，Vol.37Issue(4)：120-124,5.
产琼胶酶菌株NBRC102603发酵条件优化及酶的分离纯化
Conditions for Enzyme Production and Purification of Agarase Produced by Agarivorans albus NBRC102603
摘要
Abstract
The fermentation conditions of an agar-degrading bacterium NBRC102603 isolated from the coastal water were optimized by using orthogonal experiment.The optimum condition for agarase production consisted of peptone 5.0g/L, yeast extraet 1.25 g/L, agar 4.0g/L, 1％ inoculating quantity,rate of shaking flask with 100 mL liquid in a 500 mL flask was 150 r/min.After 48h fermentation at 28℃ ,the enzyme activity was 58.94U/mL,which was 2.08 times higher than before optimization.After a serious of purification processes, two types of purified agarases of NBRC102603 were obtained ,identified as agarase A and agarase B.Agarase A was purified 17.29-fold, with a specific activity of 870.51 U/mg; while B was purified 16.65-fold,with a specific activity of 838.39U/mg.The purified enzyme A and B appeared to be homogeneous under the inspection of SDS-PAGE, and they had molecular masses about 83.6 ku and 36.8 ku.关键词
海洋细菌/琼胶酶/正交试验/分离纯化Key words
marine baeteria/agarase/orthogonal experiment/purification引用本文复制引用
朱慧文,孙延娜,周晓龙,郝梅,郭振博,施超,朱启忠..产琼胶酶菌株NBRC102603发酵条件优化及酶的分离纯化[J].食品与发酵工业,2011,37(4):120-124,5.基金项目
国家大学创新性基金项目(101042239) （101042239）
山东大学(威海)科技立项重点项目专项基金(A10010) （威海）
