食品与发酵工业2011，Vol.37Issue(4)：194-197,4.
采用高通量微生物法和HPLC法检测猪肉中四环素和磺胺类抗生素残留
Analysis of Tetracycline and Sulfonamide Antibiotic Residues in Pig Muscle Using Throughout Microbial Assay and HPLC
摘要
Abstract
A new protocol to analyze tetracyclines and sulfonamides from pig muscle was developed. Six antibiotics, oxytetracycline, tetracycline, chlortetracycline, slfamonomethoxine, sulfamethazine, and sulfamerazine, were analyzed by microbial assays and HPLC. Muscle juice samples were screened with Bacillus sterothermophilus in 96-well microplate. At the same time, the muscles samples were extracted with Mcllvaine-EDTA buffer solutions and then were detected at 270nm and 360nm. The detection limits of microbial assay were 0.02 ～ 0. 05μg/mL. For HPLC method, the limits of detection were 0.03 ～0.06 mg/kg, and the average recoveries were 80.25 ％ ～ 118.60％.关键词
高通量微生物法/HPLC/四环素类抗生素/磺胺类抗生素Key words
throughout microbial assay/ HPLC/ tetracyclines/ sulfonamides引用本文复制引用
刘兴泉,冯震,姚蕾,李博斌..采用高通量微生物法和HPLC法检测猪肉中四环素和磺胺类抗生素残留[J].食品与发酵工业,2011,37(4):194-197,4.基金项目
浙江省钱江人才项目(2008R10015) （2008R10015）
