食品与发酵工业2011，Vol.37Issue(4)：204-208,5.
谷物中赭曲霉毒素A化学发光酶免疫分析法的建立
Development of Chemiluminescence Enzyme Immunoassay for the Determination of Ochratoxin A in Cereal
摘要
Abstract
A sensitive indirect competitive chemiluminescence enzyme immunoassay (CLEIA)for OTA was developed. The concentration of OTA causing 50％ inhibition of binding enzyme marker (IC50) was 112 pg/mL and the detection limit was 2.47 pg/mL, the mean coefficients of variation of within groups and between groups were 7.03,14.7％ respectively. The recoveries from rice and wheat samples spiked with 1.5 ～ 6 Ig/kg of OTA varied between 66.97％ and 97.96％ ,and the OTA antibody showed no cross -reactivity to the else common mycotoxins. OTA in 30 cereal samples were screened by the CLEIA, including 20 rice samples and 10 wheat samples. The results show good coefficient with commercial ELISA kit (R2 = 0. 9424). The assay was sensitive, fast and accurate, which proved to be suitable for the screening of cereal samples for the presence of OTA.关键词
赭曲霉毒素A/化学发光酶免疫分析/谷物Key words
Ochratoxin A/ CLEIA/ cereal引用本文复制引用
刘星,许杨,何庆华..谷物中赭曲霉毒素A化学发光酶免疫分析法的建立[J].食品与发酵工业,2011,37(4):204-208,5.基金项目
国家863计划项目基金(No.2007AA10Z427) （No.2007AA10Z427）
