茶叶科学2011，Vol.31Issue(3)：218-224,7.
EGCG3" Me提取与分离工艺研究
Study on Extraction and Separation Process of EGCG3"Me
摘要
Abstract
The extraction and separation process of EGCG3"Me was studied by HPLC, polyamide resin and HPTLC methods. According to the orthogonal experimental design, the optimum extracting conditions of EGCG3"Me were as follows: alcohol concentration 70％, reflux time 30 min, reflux temperature 80℃ and solid-liquid ratio 1:10. And the yield of EGCG3"Me was 0.89％. Large amount of EGCG3"Me was found in the elution of alcohol concentration 40％ and 60％. With elution of methanol: chloroform (7:2), the high purity EGCG3"Me was obtained by further separation and purification of 100～120 mesh polyamide resin.关键词
EGCG3"Me/提取/分离/HPLC/聚酰胺Key words
EGCG3"Me/ extraction/ separation/ HPLC/ polyamide分类
轻工纺织引用本文复制引用
罗正飞,金孝芳,龚正礼,梁名志,汪毅,王立波,陈继伟..EGCG3" Me提取与分离工艺研究[J].茶叶科学,2011,31(3):218-224,7.基金项目
重庆市科委项目资助(CSTC.2007AC1044) （CSTC.2007AC1044）
