食品工业科技Issue(5)：98-101,104,5.
表没食子儿茶素没食子酸酯对嗜水气单胞菌抑菌机制的研究
Study on the membrane action mechanism of EGCG against Aeromona hydrophila
摘要
Abstract
Antimicrobial effect of EGCG against Gram-negative Aeromonas hydrophila was investigated. As a result, minimal inhibitory concentration(MIC) of EGCG against Aeromonas hydrophila was 25～5Oμg/mlThe mechanism of action was further investigated by cell integrity and membrane permeabilization assays. The assay showed that EGCG obviously increased the membrane permeability, and induced the leakage of ion and macromolecules in the cytoplasm. Flow cytometric analysis revealed that EGCG can cause significant membrane damage for influx of ONPG into cells. Results indicated that EGCG killed Aeromonas hydrophila by disrupting the structure of cell membranes and caused the leakage of intracellular components from bacterial cells.关键词
EGCG/嗜水气单胞菌/抑菌机理Key words
EGCG/ Aeromonas hydrophila/ antimicrobial mechanism分类
轻工纺织引用本文复制引用
卢春霞,王洪新,吕文平,徐跑,朱健,谢骏,刘波,周群兰..表没食子儿茶素没食子酸酯对嗜水气单胞菌抑菌机制的研究[J].食品工业科技,2011,(5):98-101,104,5.基金项目
国家科技部中小企业技术创新基金项目(07C26115201947) （07C26115201947）
农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放课题(BZ2009-24). （BZ2009-24）
