食品工业科技Issue(5)：323-327,5.
肠溶包衣乳糖酶纳米微囊的制备及稳定性研究
Preparation and stability of enteric-coated nanocapsules loaded with lactase
摘要
Abstract
An oral preparation which could protect lactase from inactivation in both the stomach and intestine was prepared. Enteric-coated nanocapsules loaded with lactase were prepared by double emulsion method and physicochemically characterized. The influence of main process parameters on the lactase stability loaded in nanocapsules was investigated, and some strategic approaches were taken for protection. The lactase stability loaded in nanocapsules in the simulated gastric juice and intestinal juice were also evaluated. The main results were as follows :The higher strength of ultrasonic, the lower activity lactase had. Dichloromethane/ethyl acetate ( 1: 1 ) as solvent composition and high pressure homogenization as second emulsion process both were beneficial for the lactase stability. Protectants,such as bovine serum albumin, polyvinyl alcohol and polyethylene glycol 400, which were co-encapsulated in the inner phase with lactase,could improve the lactase stability efficiently. The prepared enteric-coated nanocapsules loaded with lactase could retain 88.97％ of its activity after 2h in the simulated gastric juice and over 95％ of its activity was retained after simulation in intestinal juice for 6h. The present data suggested that these nanocapsules could be a useful carrier for the oral administration of lactase.关键词
乳糖不耐受症/肠溶包衣/乳糖酶/纳米微囊/稳定性Key words
lactose intolerance/ enteric- coated/ lactase/ nanocapsules/ stability分类
轻工纺织引用本文复制引用
贺红军,孔保华,夏娟,姜竹茂..肠溶包衣乳糖酶纳米微囊的制备及稳定性研究[J].食品工业科技,2011,(5):323-327,5.基金项目
国家高技术研究发展计划863项目(2008AA102315) （2008AA102315）
黑龙江省教委海外学人项目(1152hq14). （1152hq14）
