食品工业科技Issue(5)：411-414,4.
离子对反相高效液相色谱法测定暗纹东方鲀肉中呈味核苷酸
Ion-pair reversed-phase high performance liquid chromatography method for determination of flavor nucleotides in Fugu obscures muscle
摘要
Abstract
An ion - pair reversed - phase high performance liquid chromatography ( HPLC ) method for the determination of flavor nucleotides such as guanosine monophosphate (GMP), inosine monophosphate (IMP),cytidine monophosphate(CMP) and adenosine monophosphate(AMP) in Fugu obscures muscle was established.Chromatographic separation was performed on a SUPELCOIL LC-18-T C18 column at the wavelength of 254nm with the mobile phase of CH3 OH :0.01 mol/L KH2 PO4 solution (5:95, V/V) at 0.8 mL/min. The average recovery rate for four flavor nucleotides ranged from 96.70％～104.47％ with the coefficient variance(CV) ranged from 1.12％～1.99％ ,their minimum detection limits were 1.42,4.10,0.67,0.91μg/mL, respectively. The method was accurate and reliable,the operation was simple and easy.关键词
呈味核苷酸/离子对反相高效液相色谱/暗纹东方鲀Key words
flavor nucleotides /ion- pair reversed- phase high performance liquid chromatography/ Fugu obscures分类
轻工纺织引用本文复制引用
龚玺,刘源,王锡昌,陶宁萍..离子对反相高效液相色谱法测定暗纹东方鲀肉中呈味核苷酸[J].食品工业科技,2011,(5):411-414,4.基金项目
上海市科委部分地方院校计划项目(08390513900) （08390513900）
"十一五"国家科技支撑计划课题(2008BAD94B09) （2008BAD94B09）
上海市教委"食品质量与安全"重点学科建设项目(J50704). （J50704）
