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双缩脲比色法分析三氯乙酸沉淀乳蛋白效果探讨

肖莲荣 任国谱 张素文

食品工业科技Issue(5):415-417,3.
食品工业科技Issue(5):415-417,3.

双缩脲比色法分析三氯乙酸沉淀乳蛋白效果探讨

Study on the effect of precipitation of milk protein by trichloroacetic acid analyzing with biuret spectrophotometry method

肖莲荣 1任国谱 1张素文1

作者信息

  • 1. 湖南亚华乳业有限公司技术中心,湖南长沙,410200
  • 折叠

摘要

Abstract

The effect of precipitation of milk protein by heating and different concentration of trichloroacetic acid (TCA) was analyzed by biuret spectrophotometry method,and it was influenced by pH that the effect precipitation of milk protein by TCA.Results showed that it was influenced by concentration of TCA that the effect of precipitation milk protein by TCA were analyzed by biuret spectrophotometry method, when using standard biuret spectrophotometry, and the concentration of TCA ≤ 15%.When TCA > 15 %, it must adjust the pH above 11.When pH≥11 ,the delicacy change of pH didn' t affect OD.There was no obvious difference between heating and not heating on the precipitation effect of milk protein by TCA.

关键词

双缩脲比色法/三氯乙酸/牛乳蛋白/沉淀效果

Key words

biuret spectrophotometry method /trichloroacetic acid/ milk protein/ precipitation effect

分类

轻工纺织

引用本文复制引用

肖莲荣,任国谱,张素文..双缩脲比色法分析三氯乙酸沉淀乳蛋白效果探讨[J].食品工业科技,2011,(5):415-417,3.

基金项目

"十一五"国家科技支撑计划项目(2006BAD04A15). (2006BAD04A15)

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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