食品工业科技Issue(5):415-417,3.
双缩脲比色法分析三氯乙酸沉淀乳蛋白效果探讨
Study on the effect of precipitation of milk protein by trichloroacetic acid analyzing with biuret spectrophotometry method
摘要
Abstract
The effect of precipitation of milk protein by heating and different concentration of trichloroacetic acid (TCA) was analyzed by biuret spectrophotometry method,and it was influenced by pH that the effect precipitation of milk protein by TCA.Results showed that it was influenced by concentration of TCA that the effect of precipitation milk protein by TCA were analyzed by biuret spectrophotometry method, when using standard biuret spectrophotometry, and the concentration of TCA ≤ 15%.When TCA > 15 %, it must adjust the pH above 11.When pH≥11 ,the delicacy change of pH didn' t affect OD.There was no obvious difference between heating and not heating on the precipitation effect of milk protein by TCA.关键词
双缩脲比色法/三氯乙酸/牛乳蛋白/沉淀效果Key words
biuret spectrophotometry method /trichloroacetic acid/ milk protein/ precipitation effect分类
轻工纺织引用本文复制引用
肖莲荣,任国谱,张素文..双缩脲比色法分析三氯乙酸沉淀乳蛋白效果探讨[J].食品工业科技,2011,(5):415-417,3.基金项目
"十一五"国家科技支撑计划项目(2006BAD04A15). (2006BAD04A15)