食品工业科技Issue(5)：168-171,175,5.
耐热β-半乳糖酶在乳酸克鲁维酵母及毕赤酵母中的表达研究
Study on expression of thermostable β-galactosidase in Kluyveromyces lactis and Pichia pastoris expression system
摘要
Abstract
Thermostable β- galactosidase gene bgaB from Geobacillus stearothermophilus were cloned into pKLAC1 and pPIC9k downstreams of the α- mating factor domain, resulting in construction of eukaryotic expression vectors of Kluyveromyces lactis and Pichia pastoris expression systems.Introduction of the linearized expression cassettes in K.lactis and P.pastoris cells was achieved by electrotransformation, and expression cassettes were inserted into the host genomes at the homology locus.Recombinant enzymes were purified by Ni2+ -NTA affinity chromatography,identified by western blot and the enzyme property was characterized.The results suggested that the recombinant thermostable β-galactosidases were capable of expression in yeast expression system and the stability was preserved well, but recombinants can not be secreted effectively.关键词
耐热β-半乳糖苷酶/乳酸克鲁维酵母/毕赤酵母/酶学性质Key words
thermostable β- galactosidase/ Kluyveromyces lactis/ Pichia pastoris/ enzyme property分类
生物科学引用本文复制引用
董艺凝,王霁昀,陈海琴,张灏,陈卫..耐热β-半乳糖酶在乳酸克鲁维酵母及毕赤酵母中的表达研究[J].食品工业科技,2011,(5):168-171,175,5.基金项目
中央高校基本科研业务费专项资金(JUSRP11017,JUSRP31002). （JUSRP11017,JUSRP31002）
