食品工业科技Issue(5)：200-202,205,4.
甘草毛状根培养体系研究
Study on culture of hairy roots of Glycyrrhiza uralensis fisch
摘要
Abstract
Some factors that affected the hairy roots growth of Glycyrrhiza uralensis fisch and Glycyrrhiza inflata bat were studied, including the source of fermentation seed, the temperature, the rotate speed and the dissolved oxygen in medium. According to the parameter data of fermentation in 5L culture and 0.5L flask cultivation,the technology of 10L stirred bioreactor was established. The results showed that: using 1‰-3‰ inoculums size,25℃,40r/min and rotated speed 0.5vvm applied air,the growth rate of the 60 days hairy roots obtained from 10L stirred bioreactor was 55～60 no less than those cultured in flask. The glycyrrhizinic acid was detected in hairy roots of Glycyrrhiza uralensis fisch by HPLC whose content was 0.1％ dry weight.关键词
甘草毛状根/体系Key words
hairy roots of Glycyrrhiza uralensis fisch/ culture分类
轻工纺织引用本文复制引用
谢丽琼,杨毅,马东建,李冠..甘草毛状根培养体系研究[J].食品工业科技,2011,(5):200-202,205,4.基金项目
新疆科技厅高技术项目(200415115). （200415115）
