食品工业科技Issue(5)：209-211,3.
海藻酸钠-壳聚糖共固定化菠萝茎蛋白酶的研究
Study on sodium alginate-chitosan immobilization of stem bromelain
高尚欣 1周冰如 1周锦丝 1李燕菲 1赵力超1
作者信息
- 1. 华南农业大学食品学院,广东广州,510000
- 折叠
摘要
Abstract
Sodium alginate and chitosan materials were fixed to the stem bromelain, and the immobilization conditions and characteristics of immobilized stem bromelain were studied.Through single factor and orthogonal experiment,the best conditions for immobilized stem bromelain were determined as follow: enzyme dosage of 0.4mg/g,3.0％ sodium alginate concentration, chitosan concentration of 1.0％, CaCl2 concentration of 5.5％,immobilized time of 90min. The relative activity of immobilized stem bromelain after repeated use four times remained above 80％. Meanwhile, the optimal pH shifted to alkalescence, the immobilized enzyme optimal temperature,thermal stability and enzymatic characteristics had been improved.关键词
海藻酸钠/壳聚糖/菠萝茎蛋白酶/固定化Key words
sodium alginate/ chitosan/ stem bromelain /immobilization分类
轻工纺织引用本文复制引用
高尚欣,周冰如,周锦丝,李燕菲,赵力超..海藻酸钠-壳聚糖共固定化菠萝茎蛋白酶的研究[J].食品工业科技,2011,(5):209-211,3.
