低pH条件下11S球蛋白热聚集现象的研究OA北大核心CSCDCSTPCD
Study on the phenomenon of 11S globulin thermal aggregation in low pH condition
以浊度、平均粒径、ζ-电位分布、11S二级结构、三级结构为评价指标,研究了低pH条件下11S球蛋白溶液的热聚集现象.热处理条件为:浓度为5mg/mL的11S在80°C加热30min.实验结果表明,11S溶液pH越低,颗粒带正电荷越多,结构越松散,在静电作用力下聚集很少,加热处理后结构更松散,溶液仍然澄清.在pH3.6时,加热处理后的ζ-电位分布结果表明,颗粒大部分都是带正电荷,基本上没有负电荷,浊度明显减小,溶液澄清.
The phenomenon of 11S globulin thermal aggregation were studied with turbidity, Z-Average, the distribution of (s')-potential, secondary structure, tertiary structure of 11S as the analysis indexes in low pH condition.The condition of the heat treatment was: 11S were heated in 80℃ for 30min at the concentration of 5mg/mL.The results showed that the lower pH value, the more electric charged it charges, the looser of its structure,there were little aggregation…查看全部>>
顾炜;杨晓泉;郭健;刘远洋
华南理工大学轻工与食品学院,食物蛋白工程研究中心,广东广州510640华南理工大学轻工与食品学院,食物蛋白工程研究中心,广东广州510640华南理工大学轻工与食品学院,食物蛋白工程研究中心,广东广州510640黑龙江八一农垦大学食品学院,黑龙江大庆163319
生物科学
11S球蛋白热聚集低pH
11S globulin thermal aggregation low pH value
《食品工业科技》 2011 (6)
水热体系中人工分子伴侣辅助大豆球蛋白可溶性聚集体的重构
83-85,3
国家自然科学基金(20776050).
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