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米糠中γ-氨基丁酸的富集及纯化工艺研究

管娜娜 张晖 王立 郭晓娜 钱海峰

食品工业科技2011,Vol.32Issue(6):292-295,4.
食品工业科技2011,Vol.32Issue(6):292-295,4.

米糠中γ-氨基丁酸的富集及纯化工艺研究

Study on enrichment and purification of γ-aminobutyric acid in rice bran

管娜娜 1张晖 1王立 1郭晓娜 1钱海峰1

作者信息

  • 1. 江南大学食品学院,江苏无锡,214122
  • 折叠

摘要

Abstract

The accumulation of GABA in rice bran by glutamate decarboxylase(GAD) ,and the purification of GABA enriched solution using cation ion exchange resin were investigated. The optimal accumulation conditions of GABA were determined as described below: the ionic strength of citric acid-disodium hydrogen phosphate buffer solution was 0.02mol/L, the reaction time was 16h. Under the optimal conditions, the concentration of GABA reached 2900mg/100g rice bran. The optimal purification conditions of GABA enriched solution also be determined as following: the optimal pH of sample solution was 2.0, the concentration of GABA enriched solution was 2.0mg/mL,concentration of eluting agent was 2.0mol/L. And the final purity of GABA reached 61.25%.

关键词

米糠/γ-氨基丁酸(GABA)/富集/纯化/离子交换

Key words

rice bran /γ- aminobutyric acid / enrichment/ purification/ ion exchange

分类

轻工纺织

引用本文复制引用

管娜娜,张晖,王立,郭晓娜,钱海峰..米糠中γ-氨基丁酸的富集及纯化工艺研究[J].食品工业科技,2011,32(6):292-295,4.

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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