食品工业科技2011，Vol.32Issue(6)：373-375,3.
山药中降糖活性成分的筛选与比较
Study on filtration and comparison of hypoglycemic bioactive components in dioscoreae rhizome
摘要
Abstract
Objective: To study the effect of extracts of dioscorea rhizome-polysaccharides, saponins and flavones to HepG2 cell with the subject of consumption glucose affection and to hypoglycemic action effect to the diabetic mice model established intra-abdominal injection of alloxan in order to compare the three components' hypoglycemic bioactive.Methods : using the D101 macroporous resin adsorption process for the ethanol extracts of dioscoreae rhizome,elution with different concentration of ethanol.Depurating get 50％ flavones content or so, and 60％ saponins,and get polysaccharides from extracts of dioscoreae rhizome by water.Then use HepG2 cell and the diabetic mice as model to study three components hypoglycemic affections.Results: It demonstrated that saponins could lead to adding consumption of glucose in unit cell and make model dose-dependent.Dioscoreae rhizome,saponins and flavones could decrease the water drinking volume of diabetic mice and level of glucose.Dioscoreae rhizome was best.It concluded that three components showed synergistic effect among dioscoreae rhizome' s dhypoglycemic bioactive base on the experiment.关键词
山药多糖/山药皂苷/山药黄酮/HepG2细胞/糖尿病Key words
dioscoreae rhizome polysaccharides/ dioscoreae rhizome saponins/ dioscoreae rhizome flavones/HepG2 cell/ diabetic分类
轻工纺织引用本文复制引用
何凤玲,叶小利,李学刚,李晓芳,浦东,李平,王小华,冯平..山药中降糖活性成分的筛选与比较[J].食品工业科技,2011,32(6):373-375,3.基金项目
重庆市攻关项目(CSTC,2010AC5007) （CSTC,2010AC5007）
重庆市科委攻关项目(CSTC 2010AC5007). （CSTC 2010AC5007）
