食品工业科技2011，Vol.32Issue(6)：388-390,3.
直接竞争酶联免疫分析方法检测猪肉中的沙丁胺醇
Determination of salbutamol residue in pig muscle by direct competitive enzyme-linked immunosorbent assay
摘要
Abstract
A direct competitive enzyme-linked immunosorbent assays(cd-ELISA)was developed for detection of salbutamol in pork using polyclonal antibody.The sensitivity (IC50)and limit of detection (IC15)of assay were 0.80μg/L and 0.06μg/L, respectively.The ELISA detection system had the linear detection of 0.06～20.00μg/L.All of the test compounds, except clenbutarol ( 100％ ) and terbutaline ( 10.5％ ), showed low cross- reactivity.Then this direct competitive ELISA could potentially be applied to the determination of salbutamol and clenbutarol.The limit of detection of this developed ELISA for salbutamol in pork was 0.6μg/kg.The average recoveries were in ranges of 85％～100％ and RSD were below 5.51％.关键词
沙丁胺醇/抗体/直接竞争ELISA/猪肉Key words
salbutamol/ antibody/ dc- ELISA/ pork分类
轻工纺织引用本文复制引用
郭柏雪,生威,余桂春,石曼,邓捷,陈文洁,王硕..直接竞争酶联免疫分析方法检测猪肉中的沙丁胺醇[J].食品工业科技,2011,32(6):388-390,3.基金项目
"十一五"科技支撑课题计划资助项目(2009BADB9B06). （2009BADB9B06）
