茶叶科学2011,Vol.31Issue(2):124-128,5.
高效液相色谱法测定茶叶中烟酸的含量
Determination of Nicotinic Acid in Tea Leaves by HPLC
裴亭1
作者信息
- 1. 江苏出入境检验检疫局工业产品检测中心,江苏,南京,210001
- 折叠
摘要
Abstract
A method to measure nicotinic acid by high performance liquid chromatography was investigated in this work. Method: using Agilent 1100 HPLC series, C18 column (4.6mm×150 mm), mobile phase of MeOH: 0.05 mol/L KH2PO4 (1 ∶ 9), with flow rate of 1.0 mL/min, and the detection wavelength at 266nm. The nicotinic acid curve was linear with R2=0.9915. The average recovery rate for Nicotinic acid was 99.38% with RSD 2.18%(n=4). Conclusion: the method is simple and has good reproducibility. It coulde be used for analysis of nicotinic acid in tea leaves.关键词
高效液相色谱/茶叶/烟酸/含量Key words
HPLC/ tea leaves/ nicotinic acid/ content分类
轻工纺织引用本文复制引用
裴亭..高效液相色谱法测定茶叶中烟酸的含量[J].茶叶科学,2011,31(2):124-128,5.