中国食品学报2011，Vol.11Issue(2)：177-184,8.
氯霉素人工抗原的合成及多克隆抗体的制备
Synthesis of Artificial Antigen of Chloramphicol and Preparation of Polyclonal Antibody
摘要
Abstract
Artificial antigen of chloramphenicol was synthesized by diazotization method. The artificial antigen were identified by UV spectrophotometer, SDS-PAGE electrophoresis and MALDI-TOFMS. Rabbits were immunized with that artificial antigens. The polyantiserum was obtained, and the antiserum titre was determined by ELSIA. The condition of ELISA was optimized. Four antibiotics were chosed to calculate the cross reactivity because of their smilar structure. The result showed conjugation ratio was 22:1 and working concentration of coating antigen was 0.25 μg/mL. The standard curve was eatablished and the value of IC50 was 0.26 μg/mL. Antiserum titre reached 128 000. The cross reactivity of 4 drugs and antibody were less than 0.1％ with high specificity.关键词
氯霉素/人工抗原/重氮化/优化Key words
chloramphenicol/ artificial antigen/ diazotization/ optimization引用本文复制引用
陆蕾,刘娜,倪庚,王琳,郑晓冬..氯霉素人工抗原的合成及多克隆抗体的制备[J].中国食品学报,2011,11(2):177-184,8.基金项目
国家高技术研究发展计划项目(No.2007AA10Z436) （No.2007AA10Z436）
